Device

Part:BBa_J92002:Design

Designed by: Justin D Smith   Group: UC Davis - BIM189C - S10   (2010-05-23)


Lead Remover and Reporter Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2924
    Illegal BamHI site found at 6729
    Illegal BamHI site found at 10785
    Illegal XhoI site found at 6417
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 829
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1165
    Illegal NgoMIV site found at 1461
    Illegal NgoMIV site found at 3375
    Illegal NgoMIV site found at 3777
    Illegal NgoMIV site found at 10321
    Illegal AgeI site found at 6696
    Illegal AgeI site found at 9536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 537
    Illegal SapI.rc site found at 2568
    Illegal SapI.rc site found at 5187
    Illegal SapI.rc site found at 6343
    Illegal SapI.rc site found at 8789


Design Notes

In order to design this part we used the standard 23. We used the NEB biobrick assembly kit for this standard.


Source

The majority of the subparts come from existing parts in the registry. We also designed a lead uptake part (Bba_J92000) which transports lead into the cell.

References